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Hyber series serum-free medium for hybridoma cells

A serum-free medium especially developed for high-density suspension culture of hybridoma cells

Hyber hybridoma cell medium is a serum-free medium independently developed by Shanghai BioEngine Sci-Tech Co., Ltd., and it is an ideal choice for culturing hybridoma cells from laboratory to large-scale suspension. It enables the rapid growth of hybridoma cells and supports the efficient expression of antibodies in hybridoma cells while providing a guarantee for the safety and reliability of the production process.

Hyber series serum-free medium for hybridoma cells

Features

A completely serum-free culture system

No ingredients of animal-derived, genetically modified plant origin or raw materials with BSE origin

No antibiotics, organic solvents, or preservatives

Containing hydrolysate, L-glutamine, hypoxanthine, and thymine nucleoside

Supporting high-density suspension culture and protein expression of hybridoma cells

Advantages

Animal-derived component-free; TSE/BSE statement available on demand;

Distinctive culture results proven in numerous studies;

Optional powder media for use in large-scale manufacturing with easy preparation procedures;

Powder media capable of a single batch size of 100,000 L;

Excellent inter-batch consistency (CPK*>1.33);

Full traceability by EU-certified ISO13485:2016 Quality Management System.*CPK: Process Capability Index. CPK>1.33 indicates good process control and small inter-batch difference of products.

Order Information
Basal Medium Feed Medium
ApplicationProduct NameCat. No.SizeFormProduct Instruction (pdf)Inquiries /Sample application
Support high-density suspension culture and efficient expression of hybridoma cells Hyber-B100S Hybridoma Cell Serum-Free Medium
EXP0111201
100 L Powder Inquiries /Sample application
EXP0111202
10 L Powder
Hyber-B100 Hybridoma Cell Serum-Free Medium
EXP0101402
1 L Liquid
ApplicationApplicationCat. No.SizeFormProduct Instruction (pdf)Inquiries /Sample application
Support high-density suspension culture and efficient expression of hybridoma cells Hyber-F100S Hybridoma Cell Serum-Free Medium EXP0111301 20 L Powder Inquiries /Sample application
EXP0111302 2 L Powder
Hyber-F100 Hybridoma Cell Serum-Free Medium EXP0100802 250 ml Liquid
*DrivingM® is a registered trademark of Shanghai BioEngine Sci-Tech Co., Ltd.
Performance
For Vaccines

Suspension acclimation of adherent hybridoma cells

The use of Hyber-B100S with gradual reduction of the serum content during continued acclimation of hybridoma cells allowed acclimation of serum-adherent cells into serum-free suspension cells. Suspension cells were individually dispersed with uniform cell size and showed a stable specific growth rate over the passages.

 

Batch culture of suspension hybridoma cells

Hyber-B100S is used for batch culture of hybridoma cells with a maximum viable cell density of 8×106 cells/ml and is more effective when used with Hyber-F100S.

 

Fed-batch usage strategy

Hyber-F100S Instructions for use of Hyber-F100S

Let us contact you

We have a professional technical team to provide high quality one-stop cell culture service from process development
and optimization, medium formulation design to medium processing and manufacturing for all biopharmaceutical companies.

If you have any question, please click "Quick Message" and leave your message,  we will reply to you as soon as possible.
If anything urgent, please call (86)21-68582660-2792.

BioEngine

3F&4F, Building 3, Lane 396, Lvzhou Ring Road, Minhang District, Shanghai, PRC

(86)21-68582660-2792

globalsales@bio-engine.com.cn

Work hours:9:00-17:00